Objective To evaluate the effectiveness of building standard nursing procedures during perioperative period of lung transplantation surgery. Methods A total of 120 patients with lung transplantation surgery were divided into two groups, control group and observation group, with 60 patients contained in each group. Patients in the control group received conventional nursing procedures during surgery. The observation group underwent the standard operation procedures during surgery. Nurses were specially trained. The surgical cooperation was performed according to the standard nursing procedures. The operation time, doctor′s satisfaction level, and the incidence of nursing defects between the two groups were compared. Results The doctor′s satisfaction levels in the observation group were significantly higher than the control group. The operation time, the incidence of nursing defects due to equipment failure in the observation group were prominently lower than the control group (all P<0.05). Conclusion The implementation of standard nursing procedures can improve the standardization of operation cooperation, nursing quality and doctor′s satisfaction levels.