Objective To retrieve, evaluate and summarize the best evidence of the whole process of bladder perfusion, so as to provide evidence-based evidence for the clinical implementation of bladder perfusion nursing practice. Methods According to the "6S" evidence model, relevant Chinese and English databases, guideline websites and professional society websites were searched to collect clinical practice guidelines, systematic reviews, evidence summary, clinical decision-making, expert consensus and randomized controlled clinical trials related to the nursing and management of the bladder perfusion process from January 2017 to December 2022. Two graduate students did the standardized integration and summary of the extracted evidence. Results A total of 8 articles were involved, including 4 guidelines, 2 expert consensus articles, and 2 randomized controlled clinical trials. Totally 28 pieces of best evidence about drug configuration, occupational exposure protection, perfusion process management, post-perfusion excretion management, adverse reactions and complications management, and continuous care management were summarized. Conclusion The best evidence summarized for the whole process management of bladder perfusion can provide evidence-based basis for clinical nursing practice, and practical management plans should be selectively formulated according to the actual situation when applying the evidence.