Objective To compare the clinical effect of continuous and intermittent airway pressure measurement on preventing Ventilator-associated Pneumonia (VAP) in mechanically ventilated patients, to provide a reference for artificial airway cuff management. Methods A total of 134 patients undergoing mechanical ventilation were averagely randomized into a continuous airway pressure measurement group and an intermittent airway pressure measurement group.The cuff pressure of the intermittent group was monitored by using an airbag pressure gauge with intervals of 4 hours, 6 hours, and 8 hours, while the continuous group was measured continuously by utilizing a continuous pressure gauge.The incidence of VAP, duration of mechanical ventilation, length of ICU stay, subglottic secretion drainage, types and duration of antibiotic use, and inflammatory markers during hospitalization were compared between the two groups. Results There weren′t significant differences between the two groups in the incidence of VAP, duration of mechanical ventilation, length of ICU stay, types and duration of antibiotic use (all P＞0.05).There weren′t significant differences in white blood cell count, C-reactive protein, procalcitonin, and 24-hour subglottic secretion between the two groups on the 1st, 3rd, and 7th day of admission (all P＞0.05). In the continuous pressure measurement group, the cuff pressure was 25 to 30 cmH2O; in the intermittent pressure measurement group, when it was measured at intervals of 4 hours, 6 hours, and 8 hours, there was significant difference in the cuff pressure (P＜0.05), and which was less than 25 cmH2O when it was mea-sured with an interval of 8 hours. Conclusion There aren′t significant differences in the incidence of VAP, duration of mechanical ventilation, length of ICU stay, types and antibiotic use when the cuff pressure measured conti-nuously or intermittently in mechanically ventilated patients.It suggests that using tapered cuffs with both pressure measurement methods can achieve similar cli-nical effects, when using the intermittent pressure measurement method, it will be better with at interval of 6 hours.