Objective To explore the current status of knowledge, attitude and practice regarding curriculum-based ideological and political education in nursing among nursing undergraduate, and to analyze its influencing factors, so as to provide a reference for nursing ideological and political education and training. Methods A self-designed questionnaire related to knowledge, attitude and practice regarding nursing curriculum-based ideological and political education was conducted among 294 nursing undergraduate. Results The total score of nursing undergraduates′ knowledge, attitude and practice regarding nursing curriculum-based ideological and political education was (91.10±10.40), among which the scores of knowledge, attitude and practice were (23.71±3.17),(28.81±4.02) and (38.57±5.06), respectively.Multiple linear regression analysis showed that the preference for nursing major, the participation of ideological and political-related courses, and the organization of activities related to ideological and political elements in colleges were the influencing factors of the total score of nursing undergraduates′ ideological and political knowledge, attitude, and practice （all P＜0.05）. Conclusion The knowledge, attitude and practice of nursing undergraduates on nursing curriculum-based ideological and political education are above the average level.In the teaching process, we should focus on students who choose nursing major involuntarily and have low interest in this field.We need to mobilize their enthusiasm to participate in the curriculum-based ideological and political education, strengthen and optimize the intensity and form of curriculum-based ideological and political construction, so as to further enhance nursing students′ level of knowledge, attitude and practice of nursing ideological and political education.