Objective To explore the application effect of the virtual and real practical teaching of nursing psychological intervention in nursing undergraduates. Methods A total of 123 nursing undergraduates were randomized into an experimental group (n=61) and a control group (n=62). In the practical teaching of psychological intervention, the combination of virtual and real practical teaching was implemented in the experimental group, while the scenario simulation teaching method was conducted in the control group. Results The score of psychological intervention practice skills, the total score of the Deep Learning Questionnaire and Class Experience Questionnaire in the experimental group were significantly higher than those of the control group (all P＜0.05). Student reflection reports showed that, the combination of virtual and real practical teaching could help them to organize professional verbal expressions for psychological intervention, choose appropriate intervention methods, establish clinical thinking of psychological intervention, enhance application capacity for psychological intervention, trigger learning interests, and downgrade the difficulty of understanding. Conclusion The combination of virtual and real practical teaching can improve nursing undergraduates′ performance of the psychological intervention, help deep learning, and enhance their class experience.