Objective To explore the effect of infusion port butterfly needle flushing in random direction versus traditional backward flushing on the residue in the port seat reservoir. Methods One hundred cancer patients with newly implanted infusion port were divided into a control group and an experimental group according to different hospitals,with 50 patients in each group. The control group took fixed butterfly needle infusion after backward flushing according to the standard, while the experimental group took fixed butterfly needle infusion after flushing in any direction. The physical infusion port was removed from the two groups at the end of chemotherapy to observe and conduct pulse experiments after separation of the base and silicone septum, so as to determine the grade of the degree of staining of the infusion port reservoir swab and the grade of fluorescent powder suspension fluorescent color. Results There weren't significant differences between the two groups in the grade of the degree of staining of the reservoir swab and the grade of fluorescent color of the phosphor suspension in the reservoir (both P>0.05). Conclusion Flushing the butterfly needle in random direction doesn′t affect the flushing effect of the infusion port reservoir, so it isn't necessary to probe the direction of the infusion port seat exit when clinical nurses opening the infusion port access, conducting accurate puncture and correct execution of the pulsatile maneuver are enough.