Objective To translate the Fertility & Infertility Treatment Knowledge Score (FIT-KS), and to test its reliability and validity among women with infertility. Methods The English version of the FIT-KS was translated into Chinese following the Brislin model, then it was conducted cultural adaptation, afterward it was used to survey 230 women with infertility to analyze its reliability and validity. Results There were 27 items in the Chinese version of the FIT-KS, the correlation coefficients among its each item were from 0.646 to 1.000 (all P<0.05); its item content validity index was 0.800-1.000; its criterion-related validity with Cardiff Fertility Knowledge Scale was 0.775. The Cronbach′s α coefficient of the Chinese scale was 0.908, and its test-retest reliability was 0.851. Conclusion The Chinese version of the FIT-KS has good reliability and validity among women with infertility in China, which provides a reliable measurement tool assessing fertility and infertility treatment knowledge.