Objective To explore the construction and operation effect of outpatient clinic on breast prosthesis wearing and guidance for patients after breast cancer surgery, and improve the quality of life of post-mastectomy breast cancer patients. Methods An outpatient clinic was set up to provide patients with breast prosthesis wearing and guidance, including breast prosthesis style and specification selection, breast prosthesis trial wearing, and breast prosthesis wearing guidance for patients. Results From March 2021 to August 2022, the clinic served 414 patients. The outpatient satisfaction rate was 99.59%. Conclusion The clinic for post-mastectomy breast prosthesis wearing and guidance can promote the body management of post-mastectomy breast cancer patients, provide patients with safe, convenient, standardized, and effective breast prosthesis selection and wearing environment and channels, and improve the quality of life of patients.