Objective To explore the application effect of ideological and political teaching mode of five-dimensional integration course in Basic Nursing. Methods A total of 223 baccalaureate nursing students in 4 natural classes enrolled in 2019 were regarded as a control group, and their counterparts (n=267) in 5 natural classes recruited in 2020 were taken as an experimental group.In the teaching of Basic Nursing, the control group was taught using traditional method, while the experimental group was taught utilizing the ideological and political teaching mode of "five-dimensional integration" course.The teaching effect was evaluated at the end of the course. Results The usual performance, operational performance, theoretical performance, overall evaluation performance and professional quality score of the experimental group were significantly higher than those of the control group （all P＜0.05）. Conclusion Implementing the "five-dimensional integration" ideological and political teaching in the course of Basic Nursing, is beneficial to improve students′ academic performance and professional quality, as well as teachers′ ideological and political awareness and teaching ability at the same time.