Objective To construct a training program based on the post requirements of clinical tutors for master of nursing specialist （MNS）, and to explore its application effects. Methods A training program based on the post requirements of clinical tutors for MNS was constructed after assessment of post training needs, literature review and experts group discussion, then it was used to train 41 MNS clinical tutors from a tertiary general hospital for one and a half years, and its application effect was evaluated after the training. Results After the training, the overall and each dimension score of competency and scientific research ability in MNS clinical tutors, their theoretical and skill examination scores were significantly higher than those before the training （all P＜0.05）. Conclusion Construction and practice of the training program based on post requirements for MNS clinical tutors, is conducive to improving the training effect, promoting their competency and scientific research ability.