Eight patients with recurrent glioblastoma were treated with intraspinal injection of chimeric antigen receptor (CAR) T cells. We provided not only routine nursing care but also intensive monitoring of cytokine release syndrome and neurotoxicity, care of skin damage, care of the lumbar cistern catheter and psychological support. All patients successfully completed the treatment, and their treatment-related complications were effectively controlled. Five patients were discharged with improvement and 3 progressed (of whom 2 were discharged and 1 had a second operation). It is suggested that individualized nursing care is helpful to patients to successfully complete the treatment.