Objective To explore the relationship among character strengths, organizational identification and job performance of clinical nurses, and to provide evidence for nursing managers to optimize human resource management and promote nurses′ job performance from the perspective of positive psychology. Methods A total of 1034 clinical nurses were selected by convenience sampling method.General data questionnaire, the Values in Action Inventory of Strengths, the Organizational Identification Questionnaire and the Job Performance Questionnaire were used for survey. Results The scores of organizational identification, character strengths and job performance were （34.82±5.57）,（163.58±16.28） and （45.54±6.44） respectively.Pearson correlation analysis showed that organizational identification, character strengths and job performance of clinical nurses were positively and pair-wisely correlated （P＜0.05 for all）.Character strengths played a partial mediating role in the relationship between organizational identification and job performance, accounting for 26.7% of the total effect. Conclusion Organizational identification can positively predict job performance of clinical nurses, and character strengths play a partial mediating role.Nursing managers should develop and apply effective intervention programs based on organizational identification and character strengths of nurses, so as to improve their work performance.