Objective To compile the Parents′ Knowledge, Attitude and Practice Questionnaire on Testicular Torsion in Children, and to test its reliability and validity, so as to provide an assessment tool for medical staff to measure parents′ knowledge, attitude and practice (KAP) of testicular torsion. Methods Based on the KAP theory the first draft of the questionnaire was formed through Delphi expert consultation and preliminary survey on the basis of literature review and semi-structured interviews.Convenience sampling method was used to select 244 children′s parents to conduct a survey.Item analysis, and reliability and validity analysis were carried out. Results There were 32 items in the questionnaire:14 items in knowledge dimension （non-scale questions）, 9 items in attitude dimension and 9 items in behavior dimension （scale questions）.The results of exploratory factor analysis showed that cumulative variance contribution rate of the two common factors (attitude and behavior) was 65.783%.The scale-level content validity index of the questionnaire was 0.860 and the content validity index of each item was between 0.800-1.000.The Cronbach′s α coefficient of the questionnaire were 0.933, the Cronbach′s α coefficients of attitude and behavior dimensions were 0.916 and 0.931, and the Spearman-Brown half-reliability of each dimension were 0.915 and 0.864 respectively. Conclusion The questionnaire has good reliability and validity, which can be used as an tool to assess parents′ KAP of testicular torsion.