Objective To develop an infection management checklist for implantable venous access port, and to explore its application effect in tumor patients. Methods An infection management checklist for implantable venous access port was designed through lite-rature review, group discussions, semi-structured interviews, expert consultation,etc.Oncology wards were randomly divided into 2 clusters, with inpatients in one cluster assigned into the control group （n=148）, who received routine nursing for implantable port, and those in the other cluster into the intervention group (n=160), who were managed with the infection management checklist. Results After the intervention, the incidence rate of port-associated infection in the intervention group was significantly lower than that in the control group; the time spent on port-associated infection treatment in the former group was significantly shorter than in the latter group (all P＜0.05). Scores of port-associated infection KAP (knowledge, belief and behavior) survey of nurses in the intervention group were higher than scores of nurses in the control group （P＜0.05 for all）. Conclusion Checklist based management could reduce the incidence rate of port-associated infection, shorten the time of infection control, and improve nurses′ KAP of port-associated infection control.