Objective To investigate the status quo and influencing factors of operating room nurses′ career calling, and to provide reference for the formulation of intervention program. Methods A total of 585 operating rooms nurses from 9 tertiary hospitals were surveyed by using the General Data Questionnaire, the Career Calling Scale, the Measure of Job Crafting (MJC), the Organizational Silence Scale and the Organizational Career Management Scale. Results The total score of career calling of operating room nurses was （39.45±4.07）.Multiple linear regression analysis showed that working years in operating room, average monthly income, average weekly overtime, job crafting, organizational silence, organizational career management were the main factors inf-luencing career calling of operating room nurses （all P＜0.05）. Conclusion Operating room nurses reported moderate level of career calling, which was affected by several factors.Nursing managers should take intervention measures targeting the influencing factors to improve nurses′ level of career calling, in an effort to improve the quality of operating room nursing service.