Objective To explore the application effect of a mobile intelligent blood collection system in blood collection work in wards. Methods Blood collection specimens in neurology wards during January to June 2020 and January to June 2021 were regarded as a control group (n=13 435) and an experimental group (n=12 378).The control group were collected routinely, while the experimental group were collected using a mobile intelligent blood collection system to optimize and standardize the existing blood collection process, and to realize the mobile, intelligent and automatic blood collection work in wards.The average blood collection time and specimen labeling error rate were compared between the two groups. Results There were significant differences in average blood collection time and specimen labeling error rate between the two groups （both P＜0.05）. Conclusion Application of the mobile intelligent blood collection system can effectively enhance nurses′ work efficiency, reduce manual errors, and finally improve hospital medical safety and patient safety.