Objective To extract and summarize the best evidence on the rehabilitation nursing and management of robot-assisted gait training for stroke patients in domestic and overseas, so as to provide a reference for developing rehabilitation schemes of robot assisted gait training suitable for stroke patients in China. Methods According to the principle of evidence-based resource "6S" model, the evidence on robot-assisted gait training for stroke patients in various databases and guideline networks in domestic and overseas was systematically searched, including clinical decision-making, guidelines, best evidence summary, and systematic evaluation.The search time was from January 1, 2016 to December 31, 2021.The overall evaluation of the literature was conducted by two researchers, the literature that met the inclusion criteria was selected, and relevant evidence was extracted. Results A total of 3 314 articles were retrieved, and 19 articles (4 guidelines, 3 evidence summary reports, 8 systematic reviews and 4 randomized controlled trials) were finally included after eliminating repetitive, irrelevant, unqualified and incomplete articles.The best evidence included 23 recommendations from 9 aspects: assessment, intervention plan, intervention timing, intervention frequency, intervention measures, pre-intervention preparation, parameter adjustment, effect evaluation and health education. Conclusion The best evidence of rehabilitation nursing on robot-assisted gait training for stroke patients summarized in this study has frontier nature and scientific nature, so it can provide corresponding evidence for clinical medical staff to implement robot gait rehabilitation training.