Objective To explore the safety and effectiveness of vacuum sealing drainage combined with continuous micro-oxygen permeation technology in nursing care of deep sternal wound infection after neonatal congenital heart disease operation. Methods A total of 13 neonates with deep sternal wound infection after operation of congenital heart disease were participants.We used vacuum sea-ling drainage combined with continuous micro-oxygen permeation technique based on thorough wound debridement and intravenous antibiotic treatment, the integrated mode of doctor-nurse dominated by specialist nurses was used to manage the wound precisely. Results The time from completion of surgery to diagnosing a deep sternal wound infection was 5-17 (8.73±3.84) days.The treatment of vacuum sealing drainage combined with continuous micro-oxygen permeation lasted 6-18 (11.92±3.48) days.The time from surgery to fully wound healing was 16-40 (25.35±7.41) days.A child with transposition of the great arteries died of cardiac arrest on the 20th day after the Switch operation, the remaining 12 children′s wounds healed well after treated with vacuum sealing drainage combined with continuous micro-oxygen permeation technique, their length of hospitalization was 24-64 (44.62±13.51) days.According to outpatient or WeChat follow-up, there wasn′t readmission due to wound-related problems. Conclusion Application of vacuum sealing drainage combined with continuous micro-oxygen permeation technique is safe and effective, which can promote deep infection wounds healing quickly.