Objective To construct the physician-nurse collaborative clinic for hemodialysis vascular access, to facilitate patients′ visit and reduce complications of vascular access. Methods The physician-nurse collaborative clinic for hemodialysis vascular access was established with three nephrologists and three hemodialysis specialist nurses. The physicians assessed function of vascular access using Doppler ultrasound, and nurses performed initial assessment, recorded the data of ultrasound monitoring, formulated plan for puncture, provided patient education, and made follow-up appointments. The effect was evaluated one year later. Results The number of patient attendance was 321, and 138 cases of fistula dysfunction were found and referred in time. Compared with usual vascular access maintenance, the first-attempt success rate of puncture of early arteriovenous fistula and the incidence of hematoma were significantly decreased after running the clinic (both P<0.05), whereas the qualified rates of arteriovenous fistula maintenance and functional exercise after patient attendance were significantly increased (both P<0.05). Patient satisfaction rate was increased to 98.4%. Specialist nurses convinced that the clinic was a good platform for professional learning and growth. Conclusion The establishment of physician-nurse collaborative clinic for hemodialysis vascular access reduces the complications of vascular access, enhances patients′ treatment experience, and enables specialist nurses to grow.