Objective To explore the effects of group positive psychological intervention on the negative emotions, psychological adaptation and well-being of parents of children with leukemia. Methods A total of 86 parents of children with leukemia hospitalized in department of pediatric were randomly divided into control group (44 cases) and intervention group (42 cases).The control group received the routine nursing program during hospitalization, while the intervention group received the group positive psychological intervention based on PERMA theory for parents on the basis of routine nursing.Hospital Anxiety and Depression Scale, Psychological Adaptation Scale and Campbell Index of Well-being Scale were used to evaluate parents of leukemia children before and after the intervention. Results After intervention, the scores of anxiety and depression in the intervention group were significantly lower than those in the control group, while the scores of psychological adaptation and well-being were significantly higher than those in the control group (all P＜0.05). Conclusion The implementation of group positive psychological intervention based on PERMA theory is conducive to improving the negative emotions of parents of children with leukemia, elevating their psychological adaptation and well-being, and promoting their physical and mental health.