Objective To explore the effect of multi-media health education rolled out in pediatric stem cell transplantation ward. Methods According to the time sequence, the accompanying family members of pediatric patients received stem cell transplantation were divided into a control group of 40 and an intervention group of 42. The control group received routine health education，while the intervention group received multi-media health education, which combined conventional health education with an education platform，and supplemented health knowledge with a special cellphone and a Wechat account. Results The intervention group was superior to the control group in terms of health knowledge scores, nursing skills scores, qualified rate of items preparation and nursing satisfaction （all P<0.05）; the anxiety scores were lower than those of the control group （P<0.05）. Conclusion Multi-media health education is helpful to improve the effect of health education, optimize doctor-patient relationship, improve family members′ nursing satisfaction. At the same time, it can effectively relieve the anxiety of family members before transplantation.