Objective To explore the practice and effect of support nurses training in fever clinic. Methods Totally, 59 fever clinic support nurses were trained. Using the Kirkpatrick Model as an evaluation tool, the support nurses were evaluated from the reaction level, learning level, behavior level and result level through training satisfaction survey, written and skill exam results, survey with the questionnaire of Medical Staff′s Ability to Respond to Infectious Disease Emergencies, and semi-structured interview. Results The overall satisfaction rate of support nurses was 96.61%; the scores of written and skill exam results, infectious disease emergency prevention ability, preparation ability and rescue ability after training were significantly higher than those before training (all P<0.05). The semi-structured interview extracted four themes:comprehensive ability was improved; training was standardized; psychological experience; training needs to be further optimized. Conclusion Carrying out training of support nurses in the fever clinic can help improve their ability to respond to infectious disease emergencies, knowledge of infectious diseases and skills.