Objective To develop a scale for knowledge, attitude, and practice (KAP) of intensive care unit staff towards enteral nutrition feeding interruption, and to evaluate the reliability and validity. Methods Based on the KAP theory, the initial scale was developed after literature review, interview and expert consultation, then 400 ICU medical and nursing staff were surveyed to test the reliability and validity. Results The scale consisted of 3 dimensions and 41 items.The cumulative variance contribution of the three dimensions was 70.341%, 70.437% and 66.550%, respectively. The Cronbach′s α coefficient of the total scale was 0.953, and the test-retest reliability was 0.795. Item level content validity index （I-CVI） ranged from 0.800 to 1.000, and the scale level-CVI/average (S -CVI/Ave) was 0.975. Conclusion The scale is valid and reliable for measuring KAP toward enteral nutrition fee-ding interruption among ICU staff.