Objective To apply an intelligent management system for specimen transport, and to improve the quality and efficiency of specimen transport. Methods We built a management team, and introduced a specimen management system for specimen transport. The effect was evaluated one year after the application. Results After application of the management system, the specimen transfer time was significantly shortened (P＜0.05), more specimens were transferred and less adverse events occurred. Conclusion The specimen management system, which effectively integrates the Internet, physical Internet of Things and big data, can significantly improve the timeliness and accuracy of specimen transfer.