Objective To explore the effect of pelvic floor muscle training combined with electrical stimulation and biofeedback on prevention of urinary incontinence in patients with orthotopic ileal neobladder. Methods Ninety-seven patients underwent radical cystectomy for bladder cancer with orthotopic ileal neobladder reconstruction were divided into a control group （n=46） and an observation group （n=51）.The control group received routine pelvic floor muscle training, while the observation group was additionally given electrical stimulation and biofeedback.The effect was evaluated after two treatment cycles. Results The cure/improvement rate, bladder volume, uroflow, detrusor pressure, voiding interval and average volume per void were significantly better in the observation group than those in the control group （all P＜0.05）. Conclusion Pelvic floor muscle training combined with electrical stimulation and biofeedback can effectively ameliorate urinary incontinence and improve urinary control ability in patients after orthotopic ileal neobladder reconstruction.