Objective To investigate the effect of nurse-navigator-led 3-level quality control inspection in ERAS program for lung cancer patients undergoing thoracoscopic resection. Methods A total of 140 lung cancer patients undergoing thoracoscopic resection were randomly divided into 2 groups of 70 cases each. The control group was given routine ERAS management, while the intervention group was subjected to a three-level quality control ERAS program led by nurse navigators, with measures including SBAR based self-assessment and mutual evaluation of charge nurses, verification and feedback from the nursing team leaders, and coordination of homogeneous ERAS path management of patients by nurse navigators. Results After the intervention, the time to get out of bed after surgery, the indwelling time of the chest tube, length of hospital stay, pains scores and hospitalization expense in the intervention group were significantly shorter and lower than those of the control group， while scores of patient satisfaction with ERAS in the former group were significantly higher than the latter (all P<0.05). Conclusion Application of nurse-navigator-led 3-level quality control inspection in ERAS program can improve the quality of ERAS implementation for lung cancer patients undergoing thoracoscopic resection.