Objective To explore the mechanism of auricular acupoints plastering on the rabbit model with gastrointestinal disorder after abdominal surgery, and to provide reference for auricular acupoint plastering treating gastrointestinal dysfunction after abdominal surgery. Methods A total of 30 SD clean rabbits were divided into a blank group, a model group and an auricular acupoint plastering group using a random number table, with 10 rabbits in each group.After successful modeling, the auricular acupoint plastering group were pressed the acupoints of stomach, large intestine and Erzhong three times a day, 30 seconds each time per acupoint, lasting 7 days.The model group received modeling surgery without plastering, and the blank group wasn′t handled. Results The first postoperative defecation and bowel sound recovery in the auricular acupoints plastering group were significantly earlier than those in the model group (P＜0.05 for both).The intervention effect, time effect and intervention〖WT6”BZ〗×〖WT〗〖WT5”BZ〗time effect of serum cortisol concentration in the three groups at 6 hours, 3 days and 7 days after surgery were significant, the serum cortisol concentration of the blank group at 6 hours and 3 days after surgery were significantly lower than those of the other two groups, and it was significantly lower in the auricular acupoint plastering group than the model group at 3 days after surgery (P＜0.05 for all).After the intervention, there were significant differences in the small intestine advancement rate, C-kit protein positive expression and iNOS activity among the three groups, and the small intestine advancement rate and C-kit protein positive expression in the model group were significantly lower than those in the other two groups, while its activity of iNOS was significantly higher, and the positive expression of C-kit protein in the auricular point plastering group was significantly lower than that in the blank group (P＜0.05 for all). Conclusion Auricular acupoint plastering can accelerate the occurring of first defecation and bowel sound recovery in rabbits after abdominal surgery, reduce their serum cortisol concentration and stress reaction, and it is conducive to enhancing the small bowel advancement rate and the positive expression of C-kit protein in the colon tissue, then improving the recovery of gastrointestinal peristalsis.