Objective To explore the effects of breast milk olfactory stimulation combined with non-nutritive sucking on feeding process and enteral nutrition complications of premature infants, and to provide reference for nutritional support of premature infants. Methods A total of 114 premature infants were randomized into a control group and an intervention group, with 57 in each group.The control group was given conventional tube feeding care, while olfactory stimulation combined with non-nutritive sucking were additionally performed in the intervention group, we dropped 0.1 mL breast milk (3 drops) under the tongue of premature infants, gave pacifier for non-nutritional sucking, and placed a large cotton swab soaking with 3 mL breast milk 2-3 cm away from the nasal cavity of premature infants for olfactory stimulation at the same time. Results After the intervention, the total oral feeding time, gastric tube retention time, parenteral nutrition maintenance time and length of hospitalization in the intervention group were significantly shorter than those in the control group (P＜0.05 for all).There were no significant differences in the time of starting oral feeding, discharged body weight, feeding intolerance rate and incidence of nosocomial infection between the two groups (P＞0.05 for all). Conclusion Olfactory stimulation of breast milk combined with non-nutritive sucking can shorten feeding transition time, gastric tube retention time, parenteral nutrition maintenance time and length of hospitalization in premature infants fed by tube feeding, and promote their growth and development.