Objective To explore the application effect of gamification learning method used in insulin standardized training for diabetes liaison nurses. Methods A total of 50 diabetes liaison nurses were randomized into a control group and an intervention group, with 25 cases in each group. The control group was trained on insulin knowledge and injection techniques using traditional theoretical teaching and skill demonstration, while the intervention group was trained using gamification learning method. Insulin-related theoretical knowledge and insulin injection pen operation technique between the two groups were compared. Results After the training, insulin theoretical knowledge score of the intervention group was significantly higher than that of the control group (P<0.01), the error rate of insulin classification knowledge was significantly lower than that of the control group (P<0.05). There were no significant differences in the score of insulin injection pen operation technique and non-standard rate of the key links of the operation process between the two groups（P>0.05 for both）. Conclusion Gamification learning method can stimulate the learning enthusiasm of diabetes liaison nurses, improve their insulin-related theoretical knowledge， and it is conducive to standardizing their insulin injection operation practice.