Objective To investigate standardized training nurses′ readiness of inter-professional learning, and to analyze its influencing factors, so as to provide reference for developing a scientific, reasonable and individualized training model for standardized training nurses. Methods A total of 200 standardized training nurses from 4 grade three hospitals in Henan province were selected using convenience sampling, then they were investigated using a general information questionnaire and the Chinese version of the Readiness of Interprofessional Learning Scale (RIPLS). Result The participants′ total score of RIPLS was (75.37±9.21) points, the average item scores of the subscale team cooperation, positive professional identity, roles and responsibilities were 4.42, 4.31 and 2.66 points respectively. Whether experienced interprofessional cooperation, the familiarity with standardized training courses, and the degree of satisfaction with the scheduling system were the influencing factors of interprofessional learning readiness among standardized training nurses (P<0.05, P<0.01). Conclusion The interprofessional learning readiness among standardized training nurses is at a medium and above level, while their understanding of nurses′ roles and responsibilities is still unclear. Nursing managers should conduct vocational education in standardized training practice, help the standardized training nurses clear their roles and responsibilities, and improve their interprofessional cooperative ability.