Objective To identify the level and factors associated with alarm fatigue in nurses in the department of anesthesiology. Methods The Nurses′ Alarm Fatigue Questionnaire was used to survey 241 nurses working in the department of anesthesiology. Results Alarm fatigue scored （20.19±7.33） points among nurses in the department of anesthesiology. Multivariate regression analysis showed that college major and night shift were influencing factors of alarm fatigue （P＜0.05,P＜0.01）. Conclusion Nurses working in the department of anesthesiology report mild to medium level of alarm fatigue.Nursing managers should strengthen on-the-job training for nurses who do not graduate from nurse anesthetist profession, and pay attention to night shift nurses, to reduce adverse events caused by alarm fatigue.