Objective To identify the level of work readiness of new graduate nurses and analyze the influencing factors. Methods Nurses with job tenure less than 1 year were selected from 5 tertiary hospitals in Hubei province and filled out a general information questionnaire and the Chinese version of the Work Readiness Scale for Graduate Nurses. Results A sample of 314 new graduate nurses reported (268.93±68.64) for work readiness. The results of logistic regression analysis showed that, work experience in the other hospitals, family support, and getting along well with classmates were predictors of work readiness （P＜0.05,P＜0.01）. Conclusion The work readiness of new graduate nurses is at a medium level and affected by multiple factors.Nursing managers should provide new graduate nurses with more support to improve their work readiness.