Objective To retrieve, evaluate and integrate the best evidence on prevention and management of hypothermia in premature infants. Methods We utilized computer-based search strategies guided by "the 6S Pyramid" to retrieve related literature, including best practice recommendations reports, guidelines, evidence summaries, systematic reviews, and expert consensus statements. Two researchers independently evaluated quality of the literature, extracted evidence and then summarized. Results Eleven publications were retrieved, including 4 best practice recommendations reports, 2 guidelines,1 evidence summary report, 3 systematic reviews, and 1 expert consensus statement. Finally, 22 pieces of best evidence were summarized, which embrace 5 themes: risk factors, assessment, temperature measurement, prevention and management interventions, and staff training. Conclusion The evidence summary provides nurses with information about prevention and management of hypothermia in preterm infants in the labor room, intra-hospital transfer process and the neonatal intensive care unit.