Objective To explore the effect of a standardized curriculum based sub-specialty training program of nursing care during cardiovascular surgery for operating room nurses. Methods A total of 48 operating room nurses having a rotation in the cardio-vascular surgery block were divided into 2 groups according to the sequential order of 2 training periods. The control group (n=23) received the traditional teaching mode of general theory teaching + demonstration of basic operating room nursing techniques.The intervention group (n=25) was subjected to a standardized curriculum based sub-specialty training program, in which, standar-dized curricula were built for the 5 sub-specialties (congenital heart disease, adult heart disease, macrovascular disease, minimally invasive cardiac surgery and end-stage cardiopulmonary disease).At the end of the block rotation, the results of knowledge exam, and skill exam in the 2 groups, as well as before-after difference in surgeon satisfaction, were compared. Results The intervention group had significantly higher scores in knowledge exam, and skill exam than the controls; surgeon satisfaction saw significant improvement from before （P＜0.01 for all）. Conclusion The standardized curriculum based sub-specialty training program of nursing care during cardiovascular surgery not only improves operating room nurses′ knowledge and skill levels, but also improves the sa-tisfaction of doctors.