Objective To explore experience and support level of nurses as second victims, to probe the association between support and professional quality of life, and to provide basis for formulation of nursing intervention for second victims. Methods Conve-nience sampling method was used to select 421 nurses who had experienced patient safety incidents.The Second Victim Experience and Support Tool (SVEST) and Professional Quality of Life Scale (Pro-QOL) were used to survey the nurses. Results The median total score of SVEST was 72.00（65.00,74.50）. SVEST was negatively correlated with compassion satisfaction, while positively correlated with burnout and secondary trauma stress dimensions of Pro-QOL （P＜0.01 for all）.Multiple linear regression analysis showed that compassion satisfaction, burnout and secondary trauma stress had predictive effects on SVEST （P＜0.05，P＜0.01）, and were able to explain 34.9% of the total variation. Conclusion The experience and support of nurses as second victims was at medium level, and had certain correlation with professional quality of life. It is suggested that nursing managers should objectively understand the expe-rience of nurses as second victims, provide necessary organizational support, advocate non-punitive culture, create a positive and healthy working environment, so as to improve nurses′ experience as second victims and their professional quality of life.