Objective To explore the influencing factors of hypoglycemia in patients after pancreatectomy, and to provide references for preventive strategies. Methods A total of 319 patients who had undergone pancreatectomy were selected. The general information, disease information and blood glucose related information of patients were collected through electronic information system. Furthermore, univariate and multivariate analysis were conducted to probe the influencing factors. Results A total of 41(12.9%) patients developed hypoglycemia after operation. Multivariate analysis showed that age ≥65 years old (OR=2.612), BMI ＜18.5 (OR=5.849), BMI between 18.5-23.9 (OR=3.584), stroke history (OR=5.617) were independent risk factors for hypoglycemia after pancreatectomy （P＜0.05，P＜0.01）. Conclusion Medical staff need to keep tabs on patients developing hypoglycemia after pancreatectomy, especially those with an advanced age, having a low to normal BMI level, or a stroke history.The conditions of the patients should be comprehensively and dynamically evaluated, and measures should be taken to prevent and handle the occurrence of hypoglycemia in a timely manner.