Objective To investigate the current situation of knowledge, belief and practice (KAP) of hospice care among hospice care workers, to analyze the influencing factors, and to provide reference for hospice care training. Methods With the self-designed Knowledge, Belief and Practice of Hospice Care Workers Questionnaire, 1 743 hospice care workers from Guangxi were surveyed. Results The KAP total among hospice care workers was （74.28±8.12）.The results of multivariate regression analysis showed that KAP totals were by workers′ monthly income, satisfaction with the intensity of the job, and hospice care management protocols （P＜0.05,P＜0.01）. Conclusion The KAP of hospice care among hospice care workers in Guangxi are at medium level, which is in need of improvement. Attention should be paid to training of hospice care workers, and to hospice care management protocols, in an effort to improve expertise of hospice care workers as well as quality of hospice care.