Objective To explore the effect of applying online rehabilitation program based on markerless motion capture technology to home-residing post-surgical breast cancer patients. Methods Totally,68 post-surgical breast cancer patients to be discharged home were divided into a control group (n=33) and an intervention group (n=35) according to random number table method.The control group received routine pre-discharge education, including the issuance of written materials for rehabilitation exercise guidance and joining the WeChat group to receive exercise reminder notification. The intervention group was subjected to an online rehabilitation exercise program which had been developed based on markerless motion capture technology.Three months into the intervention, scores of functional exercise compliance, assessment of upper limb function, shoulder range of motion, and grip strength were compared between the two groups. Results There were no significant differences in functional exercise compliance scores, upper limb function assessment scores, shoulder range of motion and grip strength between the two groups at discharge (P＞0.05 for all).Three months after discharge, the above-mentioned indexes (except grip strength) of the intervention group were significantly better than those of the control group (P＜0.05,P＜0.01). Conclusion Post-surgical breast cancer rehabilitation exercise program developed based on markerless motion capture technology could effectively increase patients′ compliance with rehabilitation exercise at home, lessen such symptoms as upper limb pain, numbness, weakness, stiffness, tingling pain, etc., boost recovery of movement of the affected shoulder joint,and facilitate rapid rehabilitation at home.