Objective To explore the effect of KeMin-SuoMa-Shang JiaQi therapy on anxiety, depression and job burnout of pediatric nurses. Methods Totally,78 pediatric nurses were selected and randomized into 2 groups. The intervention group (n=39) was treated with KeMin-SuoMa-Shang JiaQi therapy, while the control group (n=40) was subjected to supportive therapy. The 2 groups received 5 weeks of intervention, one session a week, 40-60 min each session. The Hamilton Anxiety Scale （HAMA）, Hamilton Depression Scale （HAMD） and Maslach Burnout Inventory （MBI） were used to evaluate the 2 groups before,5 weeks into and 12 weeks after the intervention, respectively. Results The total scores of HAMA, HAMD and MBI measured at various time points in the intervention group had significant differences with those in the control group （P＜0.05,P＜0.01）. Conclusion KeMin-SuoMa-Shang JiaQi could significantly alleviate anxiety and depression of pediatric nurses, reduce the sense of job burnout and improve their mental health.