Objective To optimize early mobilization scheme for patients with acute ischemic stroke based on orthogonal design. Methods The three factors ［activity intensity(B), activity frequency(C),duration of each activity (D)］ affecting early activities and their corresponding levels were matched into 9 trial schemes of early mobilization according to orthogonal design L9（33）.A total of 63 patients with acute ischemic stroke were randomized into 9 groups, with each group being subjected to one trial scheme of early mobilization.Factor A(activity initiation time) was not controlled during the trial.The changes in scores of Barthel index,fatigue severity, stroke self-efficacy, quality of life（QOL）, participation and autonomy(IPA), emotions(irritability,depression and an-xiety), and neural function at discharge and 1 month after discharge. Results Factor A(activity initiation time) had significant effect on fatigue severity level at discharge and 1 month after discharge, and QOL at 1 month after discharge (P＜0.05,P＜0.01), with A2 the optimal level.The influence of factor B(activity intensity) on Barthel index score at discharge and 1 month after discharge, fatigue severity level at discharge, QOL and IPA at 1 month after discharge was significant (P＜0.05,P＜0.01), with B3 the best level.Factor C(activity frequency) had significant effect on neural function score at discharge, and scores of Barthel index, stroke self-efficacy, QOL and IPA at 1 month after discharge (all P＜0.05), with C2 the optimal level. The 3 levels of factor D (duration of each activity) had no significant effect on every outcome indicator at discharge and 1 month after discharge (all P＞0.05). Conclusion The optimal early mobilization scheme for patients with acute ischemic stroke included the following components: the initiation of mobilization came 24-48 hours after stroke; the frequency was 2-3 times per day; the activity intensity was recommended to carry out bed and chair transfer, sitting from bed, standing and walking(or climbing up stairs) if patients′ mobilization ability permitted.