Objective To account the operation costs for insertion, maintenance and removal of implanted vascular access ports, and to provide references for healthcare administration to set appropriate prices of the technique. Methods A step allocation method of project cost was adopted to measure the operation cost for insertion, maintenance and removal of implanted vascular access ports: the direct costs were measured by labor costs, material costs and equipment depreciation costs, and indirect costs were measured by allocation of direct costs; then the cost for each step (insertion, maintenance and removal) were calculated. Results The operation costs for insertion, maintenance and removal of implanted vascular access ports were ￥（1 186.93±37.33）, ￥（55.03±1.54）, ￥（850.25±57.77） respectively, which were much higher than the current pricing standards adopted by hospitals in Shanghai. Conclusion The current pricing standards for insertion, maintenance and removal of implanted vascular access ports fail to embody investment in medical-nursing services and fail to scoop back value compensation.It is recommended that the pricing standards should be adjusted and charging for maintenance of implanted vascular access ports be included into current charging system based on the operation costs, in order to promote continuous development and supply of port services.