Objective To construct a pediatric CVC maintenance standardized knowledge base, to design the system logical function for each item of the knowledge base, to improve nurses′ evidence-based practice and to boost evidence implementation. Methods We searched for literature on pediatric CVC maintenance per evidence-based methodology and extracted the particulars regarding maintenance measures, which were coded in standardized terminology in pursuant to clinical nursing classification system.Then we held expert panel discussions with the attendance of experts specialized in catheter maintenance for revision of the knowledge base and made the items suitable for clinical workflow. When the base was finalized, we designed decision support schemes in conformity with the function of nursing decision support system. Results A knowledge base that contains 21 pieces of evidence was constructed, and a 5-solution clinical decision support scheme, which includes automatic trigger, intelligent push, template generation, automatic association, reminder/warning, was formed. Conclusion Construction of the pediatric CVC standardized maintenance knowledge base helps nurses make clinical decisions, and boost efficient evidence implementation and transmission.