Objective To explore the effect of ginger-separated moxibustion combined with five-element music therapy on patients with chronic fatigue syndrome. Methods A total of 67 patients with chronic fatigue syndrome of spleen-kidney Yang deficiency were randomly divided into an intervention group (n=33) and a control group (n=34).The control group was given routine care, while the intervention group was additionally given ginger-separated moxibustion combined with five-element music therapy.Before and four weeks into the intervention, fatigue symptom level and spleen-kidney Yang deficiency syndrome score were compared between the 2 groups. Results After the intervention, fatigue symptom scores and spleen-kidney Yang deficiency syndrome score in the intervention group had significant differences in the control group (P＜0.01 for all). Conclusion Ginger-separated moxibustion combined with five-element music therapy can effectively lower fatigue symptom scores and spleen-kidney Yang deficiency syndrome score in patients with chronic fatigue syndrome.