Objective To investigate the effect of cooperation between front-line worker supervisor and head nurse on management of the workers in the operating room. Methods We set a supervisor for assisting head nurse to manage workers in the operating room in 2019. The effect was evaluated before and after the intervention. Results After implementation of the collaborative management, the monthly quality assessment scores of the workers, environmental hygiene inspection scores, satisfaction of the medical staff and the workers were significantly improved (P<0.01 for all). Work defects were decreased. Conclusion Cooperation between front-line worker supervisor and head nurse can enhance working quality of the workers in the operating room, and also promote satisfaction of the workers and the medical staff. 