Objective To construct an open online course of Clinical Nursing of TCM on the massive open online courses (MOOCs) platform of Chinese universities and to evaluate the teaching effect. Methods Contents of the course was reconstructed following the order of selecting typical diseases for lecture delivery，selecting content modules，and selecting teaching modalities. Finally, the course consisted of 81 microlectures that taught 29 common diseases and syndromes in internal medicine, surgery, gynecology and pediatrics. A total of 286 undergraduate nursing students of Grade 2018 learned the course through online and offline blended teaching. And the course was free for public use. Results The nursing students scored (75.52±8.73) points on theoretical examination, (86.26±4.22) points on usual performance, and (81.10±7.68) on comprehensive examination. Majority of them (85.0%-94.8%) were satisfied with the teaching. A total of 1 130 persons (mainly clinical personnel and students of other universities) learned the course, of whom 104 completed the course and gained score (26 were qualified and 78 were unqualified). Conclusion The online open course of Clinical Nursing of TCM is reasonable, which in turn is conducive to improving teaching effects and public use.