Objective To investigate the readiness for evidence-based practice (EBP) regarding low molecular weight heparin (LMWH) injected subcutaneously to inpatients in department of cardiology，and to boost successful translation of evidence．Methods A total of 44 nurses who participated in the EBP project subcutaneous injection of low molecular weight heparin were selected, and filled out a self-designed general information questionnaire and the Clinical Readiness of Evidence-based Nursing Assessment (CREBNA)． Intervention measures were formulated and implemented, before the nurses were reassessed with CREBNA. Results CREBNA total and dimensions scores before and after the intervention had significant differences （P＜0.01 for all）. Conclusion Assessing EBP readiness before an EBP project and taking targeted measures could make the project run smoothly.