Objective To explore the effect of breast massage combined with acupoint stimulation on milk volume of mothers of premature infants. Methods Totally, 160 mothers separated from their preterm neonates were randomized into 4 groups (respectively receiving breast massage alone, acupoint stimulation alone, breast massage plus acupoint stimulation, and routine nursing care) of 40 cases each. Within 24 hours after delivery, according to the results of random grouping, the mothers were given corresponding guidance until the baby returned to the mother. The time to reach phase Ⅱ lactation, milk volume in the first seven days and exclusive breastfeeding rate in 30 days were compared among the four groups. Results There were significant differences in the start-up time of phase Ⅱ lactation, milk volume of the first 7 days and exclusive breastfeeding rate in 30 days among the four groups (P<0.01 for all). Conclusion Breast massage and acupoint stimulation, either alone or in combination, can accelerate the start-up time of phase Ⅱ lactation, increase milk secretion in early postpartum period and exclusive breastfeeding rate 1 month postpartum. The effect of combined use is better than breast massage alone or acupoint stimulation alone.