Objective To retrieve and summarize the best evidence on intrahospital transport of critically ill patients from the emergency department, and to provide reference for standardizing intrahospital transport. Methods Relevant evidence regarding intrahospital transport of the critically ill from the emergency department was retrieved in international and national electronic databases, then quality of the literature was assessed independently by two researchers. Data were extracted from the included papers. Results Thirteen papers were included, including 1 clinical decision-making, 3 evidence summaries, 2 guidelines, 4 systematic reviews and 3 expert opinions/consensuses.We summarized 30 pieces of best evidence, including routine management of equipment, risk assessment, preparation for transport, monitoring and treatment during transport, handover and recording, quality control and training. Conclusion Evidence summarized in this study can be used to formulate protocol for intrahospital transport of critically ill patients from the emergency department.