隔姜灸对大鼠脊髓损伤后神经源性膀胱M2、M3乙酰胆碱mRNA及P2X3受体表达的影响
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女,硕士在读,学生

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河南省高等学校重点科研项目(18A360016)


Effect of ginger-partitioned moxibustion on the expression of M2- and M3- muscarinic acetylcholine receptor mRNAs and P2X3 receptor in rat model of neurogenic bladder after spinal cord injury
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    摘要:

    目的 探讨隔姜灸改善脊髓损伤大鼠神经源性膀胱症状的作用靶点,为脊髓损伤神经源性膀胱患者临床干预提供理论基础。 方法 选取SD大鼠为实验动物,随机分组后,按照改良Allen′s法建立T10脊髓损伤模型,根据评估标准,筛选符合脊髓损伤后神经源性膀胱标准的动物模型纳入模型组和隔姜灸组;假手术组仅切除T10椎板,不破坏脊髓;各组均纳入10只SD大鼠。三组术后均给予抗感染、创口护理及Crede手法辅助排尿。隔姜灸组同时行隔姜灸治疗,即术后第1天开始,每日1次,每次每个穴位灸10 min,连续12 d。 结果 隔姜灸组治疗后嘌呤能P2X3受体的相对表达水平及M2、M3乙酰胆碱mRNA的相对表达水平显著高于模型组(均P<0.05)。 结论 隔姜灸治疗可提高脊髓损伤大鼠脊髓神经节嘌呤能P2X3受体及膀胱组织中M2、M3乙酰胆碱mRNA的表达水平,从而有利于改善神经源性膀胱功能障碍。

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    Objective To explore the target sites for improving bladder function in rat model of neurologic bladder after spinal cord injury (SCI) by employing ginger-partitioned moxibustion, and to provide a theoretical basis for clinical intervention. Methods Sprague-Dawley (SD) rat model of T10 SCI was developed through modified Allen′s method, then those met diagnosis of neurologic bladder after SCI were treated as the model group and the ginger-partitioned moxibustion group, with 10 rats in each group. Ten rats receiving removal of the T10 lamina without damage of the spinal cord were treated as the sham group. All rats in the 3 groups received anti-infection treatment, wound care and Crede manoeuvre. Meanwhile, the ginger-partitioned moxibustion group was given indirect moxibustion using ginger once a day, 10 min for each acupoint at day 1 postoperatively for 12 consecutive days. Results After treatment, the expression levels of P2X3 receptor, M2- and M3- muscarinic acetylcholine receptor mRNAs in the ginger-partitioned moxibustion group were significantly higher than those in the model group (P<0.05 for all). Conclusion Ginger-partitioned moxibustion could improve the expression levels of P2X3 receptor in spinal ganglion, M2- and M3- muscarinic acetylcholine receptor mRNAs in the urinary bladder tissue of rats with SCI, so as to ameliorate bladder function.

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闫晓,李在洺,冯晓东,白俊敏,刘承梅.隔姜灸对大鼠脊髓损伤后神经源性膀胱M2、M3乙酰胆碱mRNA及P2X3受体表达的影响[J].护理学杂志,2020,35(19):1-3+17

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  • 收稿日期:2020-04-20
  • 最后修改日期:2020-06-30
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  • 在线发布日期: 2022-09-06