Objective To investigate the status of evidence-based pain management practice among hospice nurses, and to explore its influencing factors, so as to provide reference for improving their practical level of pain management. Methods A total of 276 hospice nurses were selected from Changsha, Zhuzhou and Yiyang region of Hunan Province using convenience sampling method, then they were investigated using the questionnaire of the pain management status and the Chinese version of Carlson′s E-BPMP Prior Conditions Instrument (CPCIs). Results The hospice nurses′total score of CPCIs was 3.05±0.49, and the subscale scores of previous practice, pain problem, innovation and social system attribute were 3.48±0.85, 3.20±0.50, 2.80±0.75 and 2.47±0.83 respectively. Working institution, professional title, training experience of pain nursing, frequently initiative observation or inquiry of the pain in the dying persons, and education experience of hospice care in school were the major influencing factors of the hospice nurses′ CPCIs score, which could explain 54.5% of the total variance (P<0.05, P<0.01). Conclusion The evidence-based pain management practice in hospice nurses is at a medium level. It′s necessary to strengthen pain related training for hospice nurses, improve their evidence-based pain management practice, so as to promote the quality of hospice care.